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Fluorophore and Quencher pairs (FRET Peptides)

Fluorescence resonance energy transfer ( FRET) is a mechanism describing excited state energy transfer from the initially excited donor to an acceptor. The donor molecules typically emit at shorter wavelengths that overlap with the absorption of acceptors. The process is a distance-dependent interaction between the electronic excited states of two molecules without emission of a photon. FRET peptides are widely used as suitable substrates in enzyme studies.

Crefu Peptides can prepare below listed Fluorophore and Quencher pairs:

Fluorophore	Quencher	Excitation	Emission	Comment
Abz2-Aminobenzoyl	Dnp2,4-dinitrophenyl	320 nm	420 nm
Abz2-Aminobenzoyl	NitroTyr 3-nitro-tyrosine	320 nm	420 nm
Mca7-Methoxycoumarin-4-yl)acetyl	Dnp2,4-dinitrophenyl	325 nm	392 nm
Trp Tryptophan	Dnp2,4-dinitrophenyl	280 nm	360 nm
EDANS	Dabcyl	340 nm	490 nm
FAM	Dabcyl	492 nm	517 nm